Efficient and scalable synthesis of 2-(1&#39;H-Indole-3&#39;-Carbonyl)-thiazole-4-carboxylic acid methyl ester and its structural analogs

ABSTRACT

Methods of synthesizing 2−(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) and structural analogs thereof. The methods include condensation reactions or condensation and oxidation reactions to form the thiazoline or thiazole moiety of ITE or its structural analogs.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.15/509,722, filed Mar. 8, 2017, which is a national stage entry ofInternational Patent Application PCT/US2015/049302, filed Sep. 10, 2015,which claims priority from U.S. Provisional Application 62/049,804,filed Sep. 12, 2014. The disclosures of the aforementioned priorityapplications are incorporated herein by reference in their entirety.

FIELD OF THE INVENTION

The invention is directed to the synthesis of2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester andstructural analogs thereof.

BACKGROUND

The aryl hydrocarbon receptor (Ah receptor or AhR) is a ligand-inducibletranscription factor that mediates a number of important biological andpharmacological processes.2-(1′H-Indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE)(Song et al. 2002, U.S. Pat. No. 6,916,834) is an endogenous ligand forthe receptor. ITE can be used to study AhR-mediated biological processesand therapeutic potentials and to treat disorders such as cancer (US2012/0214853, U.S. Pat. No. 8,604,067, Wang et al. 2013, Cheng et al.2015), obesity (U.S. Pat. No. 7,419,992), and conditions related toimbalanced actions of the immune system (Quintana et al. 2010, Nugent etal. 2013).

The original ITE synthesis scheme (Grzywacz et al. 2003, U.S. Pat. No.7,002,019) provided for small-scale synthesis of ITE for initialconfirmation of its structural identification (Song et al. 2002, U.S.Pat. No. 6,916,834) and for laboratory-scale biomedical studies.However, the original synthesis scheme is not capable of efficientlyproducing ITE at levels required for clinical studies in large animalsand human subjects or therapies.

The efficiency of intra-molecular cyclization to form a thiazoline ringin the original ITE synthesis scheme (Grzywacz et al. 2003, U.S. Pat.No. 7,002,019) is extremely low and becomes even lower as its syntheticscale increases. The inefficiency of this key step severely limits theefficiency of the entire synthesis. The intra-molecular cyclization ismost probably hindered by a neighboring carbonyl group. Due to thepresence of the carbonyl group, success using other cyclizationreactions is unpredictable.

A new synthesis that efficiently forms a thiazoline or thiazole ring isneeded in order to develop an efficient and scalable process forlarge-scale production of ITE and its structural analogs.

SUMMARY OF THE INVENTION

Disclosed herein are methods of synthesizing2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE)and its structural analogs using very efficient condensations ofintermediates to form frameworks containing, for example, moieties ofindoles and thiazolines or indoles and thiazoles. The methods disclosedherein eliminate the bottleneck present in the original synthesisscheme, thereby dramatically increasing the efficiency and scalabilityof synthesis. In addition, the methods disclosed herein are safe andcontrollable, employ mild conditions for all reaction steps, and employreadily available, low-cost materials and reagents.

Disclosed herein is a method comprising condensing a compound of FormulaII:

or a salt thereof,with a compound of Formula III:

or a salt thereof,to yield a compound of Formula IV:

or a salt thereof.

In Formulas II, III, and IV, the substituents W, Y, and Z are eachindependently selected from the group consisting of oxygen (O) andsulfur (S). R₁, R₂, R₃, R₄, R₅, R₆, R₇, and R_(N) are each independentlyselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, cyano, formyl, nitro, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, halothiocarbonylthio, and—S(O)_(n)R₈ (n=0 to 2, R₈ is directly connected to S), wherein R₈ isselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, thiol, cyano, formyl, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, and halothiocarbonylthio, exceptthat R_(N) may further be selected from an amino protecting group. Thecondensing is preferably conducted in the presence of an aproticsolvent. The condensing is preferably conducted in the presence of abase.

The above-described method may optionally comprise oxidizing thecompound of Formula IV to yield a compound of Formula I:

or a salt thereof.In Formula I, the substituents are as described above for Formulas II,III, and IV.

In some versions, the oxidizing step used to yield the compound ofFormula I is conducted without substantial isolation of the compound ofFormula IV from the reaction mixture in which the compound of Formula IVwas synthesized.

In some versions, the oxidizing step used to yield the compound ofFormula I comprises adding an oxidant directly to the reaction mixtureor a diluted reaction mixture comprising the reaction mixture dilutedwith solvent.

In some versions, the method further comprises, after the condensingstep and prior to the oxidizing step, diluting the reaction mixture byan amount of at least about 2-fold.

In some versions, the method further comprises, after the condensingstep and prior to the oxidizing step, cooling the reaction mixture or adiluted reaction mixture comprising the reaction mixture diluted withsolvent from a condensation reaction temperature at which the condensingis conducted to a cooled temperature. The cooled temperature may be atleast about 10° C. lower than the condensation reaction temperature.

In some versions, the method further comprises adding an oxidant to thereaction mixture or the diluted reaction mixture when the reactionmixture or the diluted reaction mixture is at the cooled temperature.

In some versions, the oxidizing is conducted at the cooled temperature.

In some versions, the method further comprises, after the cooling,heating the reaction mixture or the diluted reaction mixture from thecooled temperature to a heated temperature and conducting the oxidizingat the heated temperature. In some versions, the heated temperature maybe at least about 10° C. higher than the cooled temperature.

Also disclosed herein is a method comprising condensing a compound ofFormula V:

or a salt thereof,with a compound of Formula VI:

or a salt thereof,to yield a compound of Formula I:

or a salt thereof.

In Formulas V, VI, and I, X is a leaving group, such as a leaving groupselected from the group consisting of chlorine (Cl), bromine (Br),iodine (I), —OS(O)₂CH₃, and —OS(O)₂C₆H₄CH₃. W, Y, and Z are eachindependently selected from the group consisting of oxygen (O) andsulfur (S). R₁, R₂, R₃, R₄, R₅, R₆, R₇, and R_(N) are each independentlyselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, cyano, formyl, nitro, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, halothiocarbonylthio, and—S(O)_(n)R₈ (n=0 to 2, R₈ is directly connected to S), wherein R₈ isselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, thiol, cyano, formyl, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, and halothiocarbonylthio, exceptthat R_(N) may further be selected from an amino protecting group.

The objects and advantages of the method will appear more fully from thefollowing detailed description of the preferred embodiment of theinvention made in conjunction with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 shows schema of exemplary methods of synthesizing the exemplarycompound 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methylester (ITE). Scheme A shows a method of synthesizing ITE from 1H-indolevia a number of intermediates. Scheme B shows methods of synthesizingITE from 1H-indol-3-yl(oxo)acetonitrile (ITE-3) in one container(“one-pot”) without purification of intermediate2-(1′H-indole-3′-carbonyl)-4,5-dihydro-thiazole-4-carboxylic acid methylester (ITE-4). Scheme C shows a method of synthesizing ITE from ITE-3via intermediate 2-(1H-indol-3-yl)-2-oxoethanethioamide (ITE-4-A2).MTBE, methyl tert-butyl ether. EtOH, ethanol. EA, ethyl acetate. TFAA,trifluroacetic anhydride. DMF, dimethylformamide. DBU,1,8-diazabicyclo[5.4.0]undec-7-ene. DCM, dicholoromethane. NBS,N-bromosuccinimide. TEA, trimethylamine. MeOH, methanol.

DETAILED DESCRIPTION OF THE INVENTION

All technical and scientific terms used herein are the same as thosecommonly used by those ordinary skilled in the art to which the presentinvention pertains unless defined specifically otherwise.

“ITE” stands for 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acidmethyl ester, an endogenous ligand for a receptor named aryl hydrocarbonreceptor (Ah receptor, or AhR).

“Structural analog” or simply “analog” of ITE refers to any compoundwith a chemical structure similar to that of ITE. Examples of structuralanalogs include compounds having the same carbon backbone but havingdifferent substitutions on the carbons in the carbon backbone or havingdifferent degrees of saturation of the carbons in the carbon backbone.

“Hydroxy”, “thiol”, “cyano”, “nitro”, and “formyl” refer, respectively,to —OH, —SH, —CN, —NO₂, and —CHO.

“Alkyl” refers to a group of one (1) to eight (8) hydrogen-saturatedcarbons connected in linear, branched, or cyclic fashion, including thecombination in linear, branched, and cyclic connectivity.

“Halo” refers to any of halogen atoms fluorine (F), chlorine (CI),bromine (Br), or iodine (I).

“Haloalkyl” refers to an alkyl substituted by one or more halo(s).

“Alkenyl” refers to a group of hydrocarbons containing two (2) to eight(8) carbons, which are linear, branched, cyclic, or in combinationthereof, with at least one carbon-to-carbon double bond.

“Haloalkenyl” refers to an alkenyl substituted by one or more halo(s).

“Alkynyl” refers to a group of hydrocarbons containing two (2) to eight(8) carbons, which are linear, branched, cyclic, or in combinationthereof, with at least one carbon-to-carbon triple bond.

“Haloalkynyl” refers to an alkynyl substituted by one or more halo(s).

“Amino protecting group” represents any group commonly used for theprotection of amino functions. Such protecting groups are discussed byP. G. M. Wuts in “Protective Groups in Organic Synthesis, 5^(th)Edition” John Wiley and Sons, Inc., New York, ©2014, ISBN-13:978-1118057483, which is incorporated herein by reference in itsentirety. Exemplary amino protecting groups include alkyl carbamates,moieties of corresponding amides, etc., such as allyl carbamate (Alloc),t-butyl carbamate (BOC), 9-fluorenylmethyl carbamate (FMOC), benzylcarbamate (Cbz), acetamide, chloroacetamide, trifluoroacetamide (TFA),phthalimide, benzylamine, triphenylmethylamine (tritylamine),benzylideneamine, p-toluenesulfonamide, tosylamide, etc.

“Amino” refers to —NR_(a)R_(b), wherein R_(a) and R_(b), both directlyconnected to the N, can be independently selected from hydrogen,deuterium, halo, hydroxy, cyano, formyl, nitro, alkyl, haloalkyl,alkenyl, haloalkenyl, alkynyl, haloalkynyl, alkoxy, haloalkoxy,thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl, thioalkanoyl,halothioalkanoyl, carbonyloxy, halocarbonyloxy, carbonylthio,halocarbonylthio, thiocarbonyloxy, halothiocarbonyloxy,thiocarbonylthio, halothiocarbonylthio, a nitrogen protective group, or—S(O)_(n)R_(c) (n=0 to 2, R_(c) is directly connected to S), whereinR_(c) is independently selected from hydrogen, deuterium, halo, amino,hydroxy, thiol, cyano, formyl, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, or halothiocarbonylthio.

“Alkoxy” refers to an alkyl connected to an oxygen atom (—O-alkyl).

“Haloalkoxy” refers to a haloalkyl connected to an oxygen atom(—O-haloalkyl).

“Thioalkoxy” refers to an alkyl connected to a sulfur atom (—S-alkyl).

“Halothioalkoxy” refers to a haloalkyl connected to a sulfur atom(—S-haloalkyl).

“Carbonyl” refers to —(CO)—, wherein (CO) indicates that the oxygen isconnected to the carbon with a double bond.

“Alkanoyl (or acyl)” refers to an alkyl connected to a carbonyl group[—(CO)-alkyl].

“Haloalkanoyl (or haloacyl)” refers to a haloalkyl connected to acarbonyl group [—(CO)-haloalkyl].

“Thiocarbonyl” refers to —(CS)—, wherein (CS) indicates that the sulfuris connected to the carbon with a double bond.

“Thioalkanoyl (or thioacyl)” refers to an alkyl connected to athiocarbonyl group [—(CS)-alkyl].

“Halothioalkanoyl (or halothioacyl)” refers to a haloalkyl connected toa thiocarbonyl group [—(CS)-haloalkyl].

“Carbonyloxy” refers to an alkanoyl (or acyl) connected to an oxygenatom [—O—(CO)-alkyl].

“Halocarbonyloxy” refers to a haloalkanoyl (or haloacyl) connected to anoxygen atom [—O—(CO)-haloalkyl].

“Carbonylthio” refers to an alkanoyl (or acyl) connected to a sulfuratom [—S—(CO)-alkyl].

“Halocarbonylthio” refers to a haloalkanoyl (or haloacyl) connected to asulfur atom [—S—(CO)-haloalkyl].

“Thiocarbonyloxy” refers to a thioalkanoyl (or thioacyl) connected to anoxygen atom [—O—(CS)-alkyl].

“Halothiocarbonyloxy” refers to a halothioalkanoyl (or halothioacyl)connected to an oxygen atom [—O—(CS)-haloalkyl].

“Thiocarbonylthio” refers to a thioalkanoyl (or thioacyl) connected to asulfur atom [—S—(CS)-alkyl].

“Halothiocarbonylthio” refers to a halothioalkanoyl (or halothioacyl)connected to a sulfur atom [—S—(CS)-haloalkyl].

One aspect of the invention comprises a method of synthesizing acompound of Formula IV by condensing a compound of Formula II with acompound of Formula III. Formula IV is:

or a salt thereof,

wherein:

-   -   W, Y, and Z are each independently selected from the group        consisting of oxygen (O) and sulfur (S); and    -   R₁, R₂, R₃, R₄, R₅, R₆, R₇, and R_(N) are each independently        selected from the group consisting of hydrogen, deuterium, halo,        amino, hydroxy, cyano, formyl, nitro, alkyl, haloalkyl, alkenyl,        haloalkenyl, alkynyl, haloalkynyl, alkoxy, haloalkoxy,        thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,        thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,        carbonylthio, halocarbonylthio, thiocarbonyloxy,        halothiocarbonyloxy, thiocarbonylthio, halothiocarbonylthio, and        —S(O)_(n)R₈ (n=0 to 2, R₈ is directly connected to S), wherein        R₈ is selected from the group consisting of hydrogen, deuterium,        halo, amino, hydroxy, thiol, cyano, formyl, alkyl, haloalkyl,        alkenyl, haloalkenyl, alkynyl, haloalkynyl, alkoxy, haloalkoxy,        thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,        thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,        carbonylthio, halocarbonylthio, thiocarbonyloxy,        halothiocarbonyloxy, thiocarbonylthio, and halothiocarbonylthio,        except that R_(N) may further be selected from an amino        protecting group.

Formula II is:

or a salt thereof,

wherein R₁-R₅, R_(N), and W are as defined above for Formula IV.

Formula III is:

or a salt thereof,

-   -   wherein R₆, R₇, Y and Z are as defined above for Formula IV.

The synthesis of a compound of Formula IV by condensing the compound ofFormula II with the compound of Formula III is shown below in Scheme 1:

The condensation of Scheme 1 is preferably conducted in the presence ofa base. The base may be any base, such as a Brønsted-Lowery base or aLewis base, but is preferably a Brønsted-Lowery base. The base ispreferably a non-nucleophilic base. Exemplary bases include1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), NaHCO₃, Na₂CO₃, triethylamine(TEA), potassium tert-butoxide, sodium tert-butoxide pyridine, potassiumcarbonate, sodium hydroxide, sodium hydride, potassium hydride,N,N-diisopropylethylamine (DIPEA), phosphazene bases, such as t-Bu-P4,lithium di isopropylamide (LDA), silicon-based amides, such as sodiumand potassium bis(trimethylsilyl)amide (NaHMDS and KHMDS, respectively),lithium tetramethylpiperidide (LiTMP), and 2,6-di-tert-butylpyridine,among others. 1,8-Diazabicyclo[5.4.0]undec-7-ene, NaHCO₃, Na₂CO₃,triethylamine are preferred. 1,8-Diazabicyclo[5.4.0]undec-7-ene andNaHCO₃ are particularly preferred.

The condensation of Scheme 1 is preferably conducted in a non-aqueoussolvent. The non-aqueous solvent is preferably an aprotic solvent.Exemplary aprotic solvents include dimethylformamide(N,N-dimethylformamide) (DMF), dimethyl sulfoxide (DMSO), pyridine,dioxane, dichloromethane, perfluorohexane, α,α,α-trifluorotoluene,pentane, hexane, cyclohexane, methylcyclohexane, decalin, carbontetrachloride, freon-11, benzene, dicholoromethane, toluene, triethylamine, carbon disulfide, diisopropyl ether, diethyl ether (ether),t-butyl methyl ether, chloroform, ethyl acetate, 1,2-dimethoxyethane(glyme), 2-methoxyethyl ether (diglyme), tetrahydrofuran (THF),methylene chloride, 2-butanone, acetone, hexamethylphosphoramide,N-methylpyrrolidinone, nitromethane, acetonitrile, sulfolane, andpropylene carbonate. Dimethylformamide, dimethyl sulfoxide, pyridine,and dioxane are preferred. Dimethylformamide, dimethyl sulfoxide, andpyridine are particularly preferred. As used herein, “solvent”encompasses any singular solvent or mixture of solvents.

The condensation of Scheme 1 is preferably conducted at a temperature offrom about 0° C. to about 85° C., such as from about 10° C. to about 75°C., from about 20° to about 70° C., from about 30° C. to about 70° C.,from about 35° C. to about 65° C., or from about 40° C. to about 60° C.

The condensation of Scheme 1 is preferably conducted for a period of atleast about 0.5, about 1, about 2, about 3, about 4 hours, or moreand/or up to about 6 hours, about 7 hours, about 8 hours, about 9 hours,about 10 hours, about 15 hours, about 20 hours, about 25 hours, about 30hours, about 40 hours or more. In some versions, the condensation ofScheme I is conducted for a period of from about 1 to about 4 hours.

The condensation of Scheme 1 is capable of reaching a percent yield ofat least about 10%, about 20%, about 30%, about 40%, about 50%, about60%, about 70%, about 80%, or about 90% and/or up to about 90%, about91%, about 95%, or more with a compound of Formula II or a compound ofFormula III as limiting reagent present in an amount of from about 0.1 gto about 10 g, about 100 g, about 250 g, or about 500 g. Thecondensation of Scheme 1 is capable of reaching a percent yield of atleast about 5%, about 10%, about 15%, about 20%, about 25%, about 30%and/or up to about 35%, about 45%, about 55%, about 65%, or more with acompound of Formula II or a compound of Formula III as limiting reagentpresent in an amount of from about 10 g to about 2 kg, about 100 g toabout 1 kg, or about 500 g.

Another aspect of the invention comprises a method of synthesizing acompound of Formula I by condensing a compound of Formula II with acompound of Formula III to generate a compound of Formula IV andoxidizing the compound of Formula IV. Formula I is:

or a salt thereof,

-   -   wherein W, Y, Z, R₁-R₇, and R_(N) are as defined above for        Formula IV.

The synthesis of a compound of Formula I by condensing a compound ofFormula II with a compound of Formula III to generate a compound ofFormula IV and oxidizing the compound of Formula IV is shown below inScheme 2:

The condensation of Scheme 2 is preferably conducted in the presence ofa base, in a solvent, at a temperature, and for a period of time asdescribed above for the condensation of Scheme 1.

The oxidation of Scheme 2 is conducted in the presence of an oxidant(oxidizing agent). Any oxidizing agent is acceptable. Exemplary oxidantsinclude air (atmosphere of the earth), 9-azabicyclo[3.3.1]nonane N-oxyl(ABNO), acetone, ammonium cerium (IV) nitrate, ammonium peroxydisulfate,2-azaadamantane N-oxyl, 9-azabicyclo[3.3.1]nonane N-oxyl,2-azaadamantane N-Oxyl (AZADO), 9-azanoradamantane N-oxyl,1,4-benzoquinone, benzaldehyde, benzoyl peroxide, bleach,N-bromosaccharin, N-bromosuccinimide, (E)-but-2-enenitrile,N-fluoro-2,4,6-trimethylpyridinium triflate,N-tert-butylbenzenesulfinimidoyl chloride, tert-butyl hydroperoxide,tert-butyl hypochlorite, tert-butyl nitrite, cerium (IV) ammoniumnitrate ((NH₄)₂Ce(NO₃)₆), chloramine-T,chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octanebis(tetrafluoroborate), 3-chloroperoxybenzoic acid, chromium compounds,chromium trioxide, Collins Reagent, Corey-Suggs Reagent, cumenehydroperoxide, copper compounds, crotononitrile, cumene hydroperoxide,1,3-dibromo-5,5-dimethylhydantoin (DBDMH),2,3-dichloro-5,6-dicyanobenzoquinone (DDQ), diethyl azodicarboxylate(DEAD), Dess-Martin periodinane, 1,8-diazabicyclo[5.4.0]undec-7-ene(DBU), diisopropyl azodicarboxylate (DIAD),1,3-diiodo-5,5-dimethylhydantoin (DIH), dimethyl sulfoxide,di-tert-butyl peroxide, 3,3′,5,5′-tetra-tert-butyldiphenoquinone (DPQ),(E)-but-2-enenitrile, ferric chloride, ferric nitrate,N-Fluoro-2,4,6-trimethylpyridinium triflate, formic acid, hydrogenperoxide, hydrogen peroxide urea adduct, hydroxy(tosyloxy)iodobenzene,hypervalent bromine compounds, hypervalent iodine compounds, iodine,iodobenzene dichloride, iodosobenzene bis(trifluoroacetate),iodosobenzene diacetate, N-iodosuccinimide, iodosylbenzene,2-iodoxybenzoicacid, iron(III), iron (V), iron (IV), Jones Reagent,Koser's Reagent, magnesium monoperoxyphthalate hexahydrate, manganesecompounds, manganese dioxide (MnO₂), manganese(IV) oxide,meta-chloroperbenzoic acid, N-methylmorpholine-N-oxide,methyltrioxorhenium, molybdenum compounds, N-bromosaccharin,N-bromosuccinimide, N-chloro tosylamide sodium salt,N-chlorosuccinimide, N-iodosuccinimide,N,N,N′,N′-tetrachlorobenzene-1,3-disulfonamide, nitric acid,nitrosobenzene, N-methylmorpholine-N-oxide,N-tert-butylbenzenesulfinimidoyl chloride, osmium tetroxide, oxalylchloride, oxone, oxygen, ozone, peracetic acid, periodic acid,peroxides, peroxy acids, phenyliodonium diacetate, pivaldehyde,potassium ferricyanide, potassium permanganate, potassiumperoxydisulfate, potassium peroxomonosulfate, 2-propanone, pyridineN-oxide, pyridinium hydrobromide perbromide, pyridinium chlorochromate,pyridinium dichromate, pyridinium tribromide, ruthenium (III-VII)compounds, Sarett Reagent, Selectfluor, selenium dioxide, sodiumbromate, sodium chlorite, sodium dichloroiodate, sodium hypochlorite,sodium nitrite, sodium perborate, sodium percarbonate, sodium periodate,sulfur, styrene, N-tert-butylbenzenesulfinimidoyl chloride, tert-butylhydroperoxide, tert-butyl hypochlorite, tert-butyl nitrite,tetrabutylammonium peroxydisulfate,N,N,N′,N′-tetrachlorobenzene-1,3-disulfonamide,2,2,6,6-tetramethylpiperidinyloxy, tetrapropylammonium perruthenate,3,3′,5,5′-tetra-tert-butyldiphenoquinone, triacetoxyperiodinane,tribromoisocyanuric acid, trichloroisocyanuric acid,1,1,1-trifluoroacetone, trifluoroacetic peracid, trimethylacetaldehyde,urea hydrogen peroxide adduct, vanadium compounds, and water, amongothers. Air, manganese dioxide, N-bromosuccinimide together with benzoylperoxide, N-bromosuccinimide together with1,8-diazabicyclo[5.4.0]undec-7-ene, and1,8-diazabicyclo[5.4.0]undec-7-ene are preferred.

The oxidation of Scheme 2 is preferably conducted in a solvent asdescribed above for the condensation of Scheme 1, except thatdimethylformamide, tetrahydrofuran, dimethyl sulfoxide, dichloromethane,and pyridine are particularly preferred.

The oxidation of Scheme 2 is preferably conducted at a temperature offrom about −10° C. to about 100° C., such as from about −10° C. to about10° C., from about 30° C. to about 90° C., or other ranges therebetween.

The oxidation of Scheme 2 is preferably conducted for a period of timeas described above for the condensation of Scheme 1. In some versions,the oxidation reaction is conducted for a period of from about 1 toabout 20 hours.

Refluxing is preferably performed when conducting the oxidation ofScheme 2.

In some versions, the oxidation of Scheme 2 is performed after purifyingthe compound of Formula IV generated in the condensation reaction andsubsequently mixing the purified compound of Formula IV with a solventand oxidant as described above.

In other versions, the oxidation of Scheme 2 is performed in a “one-pot”synthesis without substantial isolation of the compound of Formula IVgenerated in the condensation reaction from the condensation reactionmixture. In the one-pot synthesis, the oxidant may be added directly tothe condensation reaction mixture or the condensation mixture dilutedwith solvent without isolation or at least substantial isolation of anycomponent therefrom. “Substantial isolation” refers to isolation of atleast about 1%, about 2.5%, about 5%, about 10%, about 15%, about 20%,about 25%, about 30%, about 40%, about 50%, about 60%, about 70%, about80%, or about 90% or more of any given component present in thecondensation reaction mixture. Addition of any oxidant described abovefor the oxidation of Scheme 2 is acceptable. Oxidants selected from thegroup consisting of air, N-bromosuccinimide, N-bromosuccinimide togetherwith 1,8-diazabicyclo[5.4.0]undec-7-ene, manganese dioxide arepreferred.

In the one-pot synthesis, the condensation reaction mixture ispreferably cooled from the condensation reaction temperature to a cooledtemperature prior to, during, and/or just after adding the oxidantthereto. The cooled temperature is preferably at least about 5° C.,about 10° C., about 15° C., about 20° C., about 25° C., about 30° C., orabout 30° C. or more and/or up to about 45° C., about 50° C., about 60°C., about 70° C., about 80° C., about 90° C., about 100° C. or morelower than the condensation reaction temperature. The cooled temperaturemay be in a range from about −30° C. to about 30° C., such as about −20°C. to about 20° C., about −10° C. to about 10° C., or about −5° C. toabout 5° C. The oxidant is preferably added when the condensationreaction mixture is at the cooled temperature.

The condensation reaction is preferably conducted for a period of atleast about 0.5 hours, about 1 hour, about 2 hours, about 3 hours, about4 hours, about 5 hours, about 7 hours, about 10 hours, about 12 hours,about 15 hours, about 17 hours, about 20 hours or more before thecondensation reaction mixture is cooled to the cooled temperature.

The oxidation reaction may be conducted at the cooled temperature or maybe conducted at an elevated temperature after reheating the reactionmixture from the cooled temperature. The elevated temperature may be atleast about 5° C., about 10° C., about 15° C., about 20° C., about 25°C., about 30° C., or about 30° C. or more and/or up to about 45° C.,about 50° C., about 60° C., about 70° C., about 80° C., about 90° C.,about 100° C. or more greater than the cooled temperature. The elevatedtemperature may be in a range from about 10° C. to about 90° C., such asabout 20° C. to about 80° C., or about 30° C. to about 70° C.

One or more solvents suitable for conducting the oxidation reaction maybe added to the condensation reaction mixture prior to conducting theoxidation reaction. The one or more solvents may be added prior to,during, and/or just after adding the oxidant and may be added prior to,during, and/or just after the cooling. The one or more solvents arepreferably added in an amount sufficient to dilute the condensationreaction mixture by an amount of at least about 1.1-fold, about1.5-fold, about 2-fold, about 2.5-fold, about 5-fold, about 10-fold,about 15-fold, about 20-fold, about 25-fold, about 30-fold, or moreand/or up to about 20-fold, about 30-fold, about 40-fold, about 50-fold,about 60-fold, about 70-fold, about 80-fold, about 90-fold, about100-fold or more. The one or more solvents may comprise any one orcombination of solvents described above for the condensation ofScheme 1. One or more solvents selected from the group consisting ofdicholoromethane, pyridine, and dimethylformamide are preferred.

When N-bromosuccinimide with 1,8-diazabicyclo[5.4.0]undec-7-ene is usedas the oxidant, the oxidation in one-pot synthesis is preferablyconducted at a temperature of from about −10° C. to about 10° C., suchas about 0° C., for a period of from about 0.5 hours to about 2 hours,such as about 1 hour. When manganese dioxide is used as the oxidant, theoxidation in one-pot synthesis is preferably conducted at a temperatureof from about 30° C. to about 50° C., such as about 40° C., for a periodof from about 4 to about 8 hours, such as about 6 hours. When air isused as the oxidant, the oxidation in one-pot synthesis is preferablyconducted at a temperature of from about 50° C. to about 90° C., such asfrom about 60° C. to about 80° C., for a period of from about 0.5 toabout 20 hours, such as from about 2 hours to about 12 hours.

Another aspect of the invention comprises a method of synthesizing acompound of Formula I by condensing a compound of Formula V with acompound of Formula VI. Formula V is:

or a salt thereof,

-   -   wherein R₁-R₅, R_(N), W, and Y are as defined above for Formula        IV.

Formula VI is:

or a salt thereof,

-   -   wherein R₆, R₇, and Z are as defined above for Formula IV, and X        is a leaving group.

The leaving group represented by X may be selected from the groupconsisting of chlorine (CI), bromine (Br), iodine (I), —OS(O)₂CH₃(mesylate, OMs), and —OS(O)₂C₆H₄CH₃ (tosylate, OTs), among others.

The synthesis of a compound of Formula I by condensing a compound ofFormula V with a compound of Formula VI is shown below in Scheme 3:

The condensation of Scheme 3 may be conducted in a solvent comprising aprotic solvent, an aprotic solvent, or a mixture of a protic solvent andan aprotic solvent. The presence of a protic solvent is preferred. Insome versions, the protic solvent comprises an alcohol. The alcohol insome versions is an aliphatic alcohol. The aliphatic alcohol may be astraight or branched short-chain alcohol (1-3 carbons), a straight orbranched medium-chain alcohol (4-7 carbons), a straight or branchedlong-chain alcohol (8-21 carbons), or a straight or branched verylong-chain alcohol (22 or more carbons). Exemplary alcohols includemethanol, ethanol, n-butanol, isopropanol, phenol,2,2,2-trifluoroethanol, ethylene glycol, glycerol, etc. In someversions, the protic solvent comprises an acid. The acid may comprise anorganic acid. The organic acid may comprise a carboxylic acid, asulfonic acid, or other acidic groups. Exemplary organic acids includeformic acid, acetic acid, propionic acid, butyric acid, valeric acid,caproic acid, oxalic acid, lactic acid, malic acid, citric acid, benzoicacid, carbonic acid, and trifluoroacetic acid, among others. Otherprotic solvents include nitromethane, amines or alkyl amines such asdiethyl amine, butyl amine, and propyl amine, ammonia, amides such asformamide, and water, among others. Various solvents or solventcombinations are suitable, such as an alcohol alone, an alcohol withwater, an alcohol with an acid, an acid alone, an acid with water, anaprotic solvent alone, an aprotic solvent with an alcohol, an aproticsolvent with an acid, an aprotic solvent with an alcohol and an acid, anaprotic solvent with water, etc. Exemplary solvents include ethanol,methanol, methanol together with water, methanol together with aceticacid, acetic acid, isopropyl alcohol, dioxane, dioxane together withmethanol, dioxane together with water, acetonitrile, ethyl acetatetogether with dimethylformamide, and ethyl acetate together withtrimethylamine.

The condensation of Scheme 3 is preferably conducted at a temperature offrom about 0° C. to about 80° C., such as from about 5° C. to about 75°C., from about 10° to about 70° C., from about 15° C. to about 75° C.,or from about 20° C. to about 60° C. In some versions, the condensationof Scheme 3 is conducted at a temperature of from about 0° C. to about40° C., such as from about 10° C. to about 30° C., from about 15° C. toabout 25° C., or about 20° C. In some versions, the condensation ofScheme 3 is conducted at a temperature of from about 40° C. to about 80°C., such as from about 50° C. to about 70° C., from about 55° C. toabout 65° C., or about 60° C.

The condensation of Scheme 3 is preferably conducted for a period of atleast about 0.5, about 1, about 2, about 3, about 4 hours, or moreand/or up to about 6 hours, about 7 hours, about 8 hours, about 9 hours,about 10 hours, about 15 hours, about 20 hours, about 25 hours, about 30hours about 40 hours or more. In some versions, the condensation ofScheme 3 is conducted for a period of from about 0.5 to about 4 hours,such as from about 1 to about 3 hours.

Refluxing is preferably performed when conducting the condensing ofScheme 3.

The condensation of Scheme 3 is capable of reaching a percent yield ofat least about 10%, about 20%, about 30%, about 40%, about 50%, about60%, about 70%, about 80%, or about 90% and/or up to about 90%, about91%, about 95%, or more with a compound of Formula V or a compound ofFormula VI as limiting reagent present in an amount of from about 0.1 gto about 10 g, about 100 g, about 250 g, or about 500 g. Thecondensation of Scheme 3 is capable of reaching a percent yield of atleast about 5%, about 10%, about 15%, about 20%, about 25%, about 30%and/or up to about 35%, about 45%, about 55%, about 65%, or more with acompound of Formula V or a compound of Formula VI as limiting reagentpresent in an amount of from about 10 g to about 2 kg, about 100 g toabout 1 kg, or about 500 g.

The elements and method steps described herein can be used in anycombination whether explicitly described or not.

The methods disclosed herein can comprise, consist of, or consistessentially of the essential elements and limitations of the methoddescribed herein, as well as any additional or optional ingredients,components, or limitations described herein or otherwise useful insynthetic organic chemistry.

All combinations of method steps as used herein can be performed in anyorder, unless otherwise specified or clearly implied to the contrary bythe context in which the referenced combination is made.

As used herein, the singular forms “a,” “an,” and “the” include pluralreferents unless the content clearly dictates otherwise.

Numerical ranges as used herein are intended to include every number andsubset of numbers contained within that range, whether specificallydisclosed or not. Further, these numerical ranges should be construed asproviding support for a claim directed to any number or subset ofnumbers in that range. For example, a disclosure of from 1 to 10 shouldbe construed as supporting a range of from 2 to 8, from 3 to 7, from 5to 6, from 1 to 9, from 3.6 to 4.6, from 3.5 to 9.9, and so forth.

All patents, patent publications, and peer-reviewed publications (i.e.,“references”) cited herein are expressly incorporated by reference tothe same extent as if each individual reference were specifically andindividually indicated as being incorporated by reference. In case ofconflict between the present disclosure and the incorporated references,the present disclosure controls.

It is understood that the invention is not confined to the particularconstruction and arrangement of parts herein illustrated and described,but embraces such modified forms thereof as come within the scope of theclaims.

EXAMPLES

The following examples show methods of synthesizing2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE)as a model compound of Formula I. The examples are presented withreference to FIG. 1.

Example 1

Example 1 shows a method of synthesizing ITE from 1H-indole via a numberof intermediates, as depicted in Scheme A of FIG. 1.

Intermediate 1 (ITE-1): 1H-indol-3-yl(oxo)acetyl chloride

1H-Indole (50 g, 0.43 mol.) and methyl tert-butyl ether (MTBE, 375 mL)were added to a three-necked round-bottom flask under stirring. Thesolution was cooled to −10° C., and then oxalyl chloride (56.9 g, 0.45mol., 1.05 eq.) was added drop-wise while keeping the temperaturebetween −10° C. and −5° C. The reaction mixture was then warmed to roomtemperature (−20° C.) and stirred at ˜20° C. for 1 hour. Petroleum ether(PE, 375 mL) was added to the reaction mixture. The suspension wasstirred at ˜20° C. for 30 min. and then filtered. The filter cake waswashed with PE (100 mL) and solvents in the cake were evaporated to give108 g of product as a yellow solid. LC/MS: 208.6[M+1]

Intermediate 2 (ITE-2): 2-(1H-indol-3-yl)-2-oxoacetamide

ITE-1 (108 g, 0.52 mol.) was added portion-wise to a solution ofconcentrated ammonia (25%, w % in water, 354 g, 5.2 mol., 10 eq.) inethanol (EtOH, 540 mL) at −5 to 14° C. After stirring for 2 hours at −5to 14° C., the mixture was added to water (540 mL) and stirred at 20° C.for 30 min. The reaction mix was then filtered and the filter cake waswashed with water (108 mL). The solvents in the cake were evaporated togive 68.5 g of product as an off-white solid (yield: 84.7%, two stepsfrom 1H-indole). LC/MS: 189.1[M+1]

Intermediate 3 (ITE-3): 1H-indol-3-yl(oxo)acetonitrile

Pyridine (95.5 g, 1.21 mol., 3 eq.) as added to a solution of ITE-2(68.5 g, 0.36 mol.) in ethyl acetate (EA, 1,000 mL) at 20° C. This wasfollowed by addition of trifluoroacetic anhydride (TFAA, 126.8 g, 0.6mol., 1.5 eq.) drop-wise at about 5-18° C. (room temperature, ˜20° C.,is acceptable) over 30 min. The mixture was stirred at about 5-18° C.(room temperature, ˜20° C., is acceptable) for 1.5 hours, quenched withsaturated aqueous sodium bicarbonate (700 mL), and stirred at 20° C. for10 min. After a phase separation, the aqueous layer was extracted withEA (2×350 mL). The combined EA layers were washed with 0.5 Nhydrochloric acid (2×350 mL) and then with saturated brine (350 mL). Theorganic layer was then dried over anhydrous Na₂SO₄ and concentrated togive 59 g of product as a pale brown solid (yield: 95.3%). LC/MS:171.1[M+1]

Intermediate 4 (ITE-4):2-(l′H-indole-3′-carbonyl)-4,5-dihydro-thiazole-4-carboxylic acid methylester

ITE-3 (1 g, 5.88 mmol.), L-cysteine methyl ester hydrochloride (1.01 g,5.88 mmol., 1 eq.), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU, 90 mg,0.587 mmol., 0.1 eq.), and N,N-dimethylformamide (DMF, 3 mL) were addedto a three-necked round-bottom flask under stirring. After stirring at40° C. for 1.5 hours, the reaction mixture was cooled to roomtemperature (˜20° C.), and 30 mL of 1 N cold aqueous hydrochloric acidwas added drop-wise under stirring. The suspension was filtered. Thefilter cake was washed with water (3×20 mL), and solvents in the cakewere evaporated to give 1.56 g of product as a pale brown solid (yield:90.63%, ITE-4 and ITE total). Dimethyl sulfoxide (DMSO), pyridine, anddioxane were also tested as solvents for the reaction with dioxaneperforming the worst overall. Other bases such as NaHCO₃, Na₂CO₃, andtriethylamine (TEA) were tested and similar yields were obtained.Reaction temperatures of 25-28° C., 40-45° C., and 60-65° C. were testedand 40-45° C. was found to be optimal. Reaction durations of 1, 2, and 4hours were tested and the results were similar except that the traceamount of starting material was still detectable in the 1 hour reaction.¹HNMR (400 MHz, d6-DMSO) δ 12.32 (brs, 1H), 8.63 (d, J=3.6 Hz, 1H), 8.19(dd, 1=6.8 Hz, 2.8 Hz, 1H), 7.55 (dd, J=6.4 Hz, 2.0 Hz, 1H), 7.31-7.25(m, 5.66 (dd, J=10.4 Hz, 8.4 Hz, 1H), 3.76 (s, 3H), 3.67 (dd, J=11.6,8.4 Hz, 1H), 3.52 (dd, J=10.4, 11.6 Hz, 1H). LC/MS: 289.1[M+1]

The final product (ITE):2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester

Active manganese dioxide (1.51 g, 17.34 mmol., 5 eq.) was added to asolution of ITE-4 (1 g, 3.47 mmol.) in THF (tetrahydrofuran, 10 mL).After refluxing for 4 hours, the mixture was cooled to room temperature(˜20° C.) and filtered through Celite. The filter cake was washed with20 mL of hot THF (50-70° C.). The combined filtrates were concentratedin reduced pressure to give 0.84 g of product as a pale yellow solid(yield: 84.59%). Dichloromethane (DCM) and pyridine were also tested.DCM was not preferred due to the low solubility of ITE in the solvent.Pyridine was not preferred, especially when air was used as an oxidantdue to its volatility. The air (atmosphere of the earth), manganesedioxide (MnO₂), N-bromosuccinimide (NBS) together with benzoyl peroxide(BPO), NBS together with 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), andDBU alone were tested as oxidant/s. The air and MnO₂ yielded betterresults with the former being more efficient. The reaction at 40° C. wasalso tested but refluxing shortened the reaction time. Durations of 1,4, and 20 hours for the oxidation reaction were tested with 1 hour beingnot complete. ¹HNMR (400 MHz, DMSO-d6) δ12.38 (brs, 1H), 9.09 (s, 1H),8.86 (s, 1H), 8.31-8.29 (m, 1H), 7.60-7.58 (m, 1H), 7.33-7.28 (m, 2H),3.91 (s, 3H). LC/MS: 287.1[M+1]

Demonstration at a Scale of 0.5 kg

For large-scale synthesis of about 0.5 kg ITE from 1-H-indole, all stepsof the process were similar to that described above for small-scalesynthesis except for reagent amounts and the parameters described below.Maintaining temperature between −10° C. and −5° C. was noted as beingimportant for reducing impurities when adding oxalyl chloride to thereaction of producing intermediate I (ITE-1). Three volumes ofdimethylformamide (DMF) were used for the condensation reaction toproduce intermediate 4 (ITE-4). The air (atmosphere of the earth) wasused as an oxidant in the oxidation reaction to produce the finalproduct (ITE) to further increase efficiency. The air was bubbledthrough the ITE-4 in 10 volumes of DMF at a flow rate of ˜3 L/min whilestirring at 80° C. for 10 hours. After cooling to room temperature, thereaction was added dropwise to 50 volumes of ice-cold water whilestirring. The suspension was filtered. The filter cake was washed threetimes with 5 volumes of water each and then dried. The product wasfurther purified by trituration in 5 volumes of methanol in reflux for30 min. and filtration after cooling down to room temperature. Dimethylsulfoxide (DMSO) was also tested in the oxidation reaction but performedworse than DMF. The oxidation reaction was tested at 60° C. but tooklonger to complete than that at 80° C. After the cyclization to makeintermediate 4 (ITE-4) in DMF, a direct oxidation with air without workup (purification of ITE-4) was also tested at 60 and 80° C. for 2 to 12hours but the impurities were higher than with the step-by-stepprocedure. The overall yield from indole to the final product (ITE) wasaround 30%, and the purity of the final product was 98.5% by HPLC (λ=214nm). One batch of 490 g and another of 622 g were produced.

Example 2

Example 2 shows methods of synthesizing ITE from1H-indol-3-yl(oxo)acetonitrile (ITE-3) in one container (“one-pot”)without purification of intermediate2-(1′H-indole-3′-carbonyl)-4,5-dihydro-thiazole-4-carboxylic acid methylester (ITE-4), as depicted in Scheme B of FIG. 1. The process ispresented in two sets of conditions.

Example 2A—The Final Product (ITE):2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester

ITE-3 (1 g, 5.88 mmol.), L-cysteine methyl ester hydrochloride (1.01 g,5.88 mmol.), pyridine (5 mL) and 1,8-diazabicyclo[5.4.0]undec-7-ene(DBU, 90 mg, 0.587 mmol.) were added to a three-necked round-bottomflask under stirring. After stirring at 40° C. for 2 hours, the reactionmixture was diluted with dichloromethane (DCM, 140 mL), then cooled to0°. To the mixture was added DBU (1.79 g, 1.18 mmol.), followed byN-bromosuccinimide (NBS, 1.15 g, 6.46 mmol.) portion-wise. Afterstirring at 0° C. for 1 hour, the mixture was quenched with IN aqueoushydrochloric acid (100 mL) and extracted with DCM (20 mL) twice. Thecombined DCM layers were washed with IN aqueous hydrochloric acid (50mL) and brine (50 mL), dried over anhydrous Na₂SO₄, and concentrated togive 1.71 g of crude product as a pale yellow solid (yield: 86.9%).Dimethyl sulfoxide (DMSO) and NaHCO₃ were tested as solvent and base,respectively, for the condensation part of the reaction, and pyridineand DBU generated less impurity. A temperature for the condensation partof the reaction at 60° C. for the duration of 12 hours yieldedcomparable results.

Example 2B—The Final Product (ITE):2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester

ITE-3 (1 g, 5.88 mmol.), L-cysteine methyl ester hydrochloride (1.01 g,5.88 mmol.), pyridine (5 mL) and 1,8-diazabicyclo[5.4.0]undec-7-ene(DBU, 90 mg, 0.587 mmol.) were added to a three-necked round-bottomflask under stirring. After stirring at 40° C. for 2 hours, the reactionmixture was diluted with dichloromethane (DCM, 140 mL), then cooled to0° C. To the mixture was added pyridine (40 mL), followed by activemanganese dioxide (MnO₂, 5.1 g, 58.76 mmol.). The mixture was stirred at40° C. for 6 hours. The mixture was then cooled to room temperature(˜20° C.) and filtered through Celite. The filter cake was washed with20 mL of hot THF (50-70° C.). The filtrate was concentrated to give 1.64g of crude product as a pale yellow solid (yield: 79.83%).

Example 3

Example 3 shows a method of synthesizing ITE from ITE-3 via intermediate2-(1H-indol-3-yl)-2-oxoethanethioamide (ITE-4-A2), as depicted in SchemeC of FIG. 1.

Intermediate 4-A2 (ITE-4-A2): 2-(1H-indol-3-yl)-2-oxo-thioacetamide

To a solution of ITE-3 (1 g, 5.88 mmol.) in pyridine (10 mL) at 60° C.was added triethylamine (TEA, 654 mg, 6.46 mmol., 1.1 eq.), followed byammonium sulfide solution (22%, w % in water, 3.64 g, 11.8 mmol., 2 eq.)drop-wise over 20 min. After stirring at 60° C. for 1.5 hours, thereaction mixture was diluted with IN aqueous hydrochloric acid (50 mL)and ethyl acetate (EA, 50 mL). After a phase separation, the aqueouslayer was extracted with EA (2×20 mL). The combined EA layers werewashed with brine (50 mL), dried over anhydrous Na₂SO₄, and concentratedto give 1.3 g of crude product as a pale brown solid (yield: 93.9%).¹HNMR (400 MHz, DMSO-d6) δ 12.15 (brs, 1H), 10.20 (brs, 1H), 10.02 (brs,1H), 8.18 (d, J=3.2 Hz, 1H), 8.13 (dd, J=6.4 Hz, 2.4 Hz, 1H), 7.53-7.51(m, 1H), 7.28-7.23 (m, 2H). LC/MS: 205.1[M+1]

Methyl Bromopyruvate

Methyl 2-oxopropanoate (50 g, 0.49 mol.) was added acetic acid (HOAc,200 mL). Bromine (47 g, 0.59 mol.) was then added drop-wise at roomtemperature (RT, ˜20° C.) over 50 min. The reaction was then stirredovernight at RT. The reaction was used directly in the next step.

The Final Product (ITE):2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester

ITE-4-A2 (100 mg, 0.5 mmol.) and the methyl bromopyruvate (108 mg, 0.6mmol.) were added to methanol (MeOH, 5 ml). The reaction was stirred at60° C. for 3 hours. The reaction mixture was poured into ice-cold water,and the solid was filtered and washed with water. The crude product wasrecrystallized in MeOH to give 75 mg of ITE (yield: 52%). Reactionconditions such as ethanol at 60° C., methanol together with water at20° C., methanol together with acetic acid at reflux, acetic acid at 60°C., isopropyl alcohol at 60° C., dioxane at 20° C., dioxane togetherwith methanol at 20° C., dioxane together with water at 20° C.,acetonitrile at 20° C., ethyl acetate (EA) together withdimethylformamide (DMF) at 20° C., and EA together with triethylamine(TEA) at 20° C. were tested. While reaction in ethanol offers the bestresult, an ethyl ester of ITE analog, instead of methyl ester (ITE), isproduced in addition to ITE and further manipulations are needed toconvert the ethyl ester to ITE. Durations of 1, 3, and 4 hours weretested, and 1 to 3 hours was preferred.

CITED REFERENCES

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1-20. (canceled)
 21. A compound of Formula II, or a salt thereof:

wherein W is selected from the group consisting of oxygen (O) and sulfur(S); and R₁, R₂, R₃, R₄ R₅ and R_(N) are each independently selectedfrom the group consisting of hydrogen, deuterium, halo, amino, hydroxy,cyano, formyl, nitro, alkyl, haloalkyl, alkenyl, haloalkenyl, alkynyl,haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl,haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, halothiocarbonylthio, and—S(O)_(n)R₈ (n=0 to 2, R₈ is directly connected to S), wherein R₈ isselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, thiol, cyano, formyl, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, and halothiocarbonylthio.
 22. Thecompound of claim 21, wherein W is oxygen (O).
 23. A compound of FormulaV, or a salt thereof:

wherein W and Y are each independently selected from the groupconsisting of oxygen (O) and sulfur (S); and R₁, R₂, R₃, R₄, R₅, andR_(N) are each independently selected from the group consisting ofhydrogen, deuterium, halo, amino, hydroxy, cyano, formyl, nitro, alkyl,haloalkyl, alkenyl, haloalkenyl, alkynyl, haloalkynyl, alkoxy,haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,carbonylthio, halocarbonylthio, thiocarbonyloxy, halothiocarbonyloxy,thiocarbonylthio, halothiocarbonylthio, and —S(O)_(n)R₈ (n=0 to 2, R₈ isdirectly connected to S), wherein R₈ is selected from the groupconsisting of hydrogen, deuterium, halo, amino, hydroxy, thiol, cyano,formyl, alkyl, haloalkyl, alkenyl, haloalkenyl, alkynyl, haloalkynyl,alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,carbonylthio, halocarbonylthio, thiocarbonyloxy, halothiocarbonyloxy,thiocarbonylthio, and halothiocarbonylthio.
 24. The compound of claim23, wherein W and Y are each oxygen (O).
 25. The compound of claim 23,wherein W and Y are oxygen (O) and sulfur (S), respectively.
 26. Amethod of synthesizing a compound, comprising condensing a compound ofFormula II to yield a framework containing an indole and thiazoline or aframework containing an indole and thiazole, wherein: Formula II is:

or a salt thereof, W is selected from the group consisting of oxygen (O)and sulfur (S); and R₁, R₂, R₃, R₄, R₅, are each independently selectedfrom the group consisting of hydrogen, deuterium, halo, amino, hydroxy,cyano, formyl, nitro, alkyl, haloalkyl, alkenyl, haloalkenyl, alkynyl,haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl,haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, halothiocarbonylthio, and—S(O)_(n)R₈ (n=0 to 2, R₈ is directly connected to S), wherein R₈ isselected from the group consisting of hydrogen, deuterium, halo, amino,hydroxy, thiol, cyano, formyl, alkyl, haloalkyl, alkenyl, haloalkenyl,alkynyl, haloalkynyl, alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy,alkanoyl, haloalkanoyl, thioalkanoyl, halothioalkanoyl, carbonyloxy,halocarbonyloxy, carbonylthio, halocarbonylthio, thiocarbonyloxy,halothiocarbonyloxy, thiocarbonylthio, and halothiocarbonylthio.
 27. Themethod of claim 26, wherein the condensing is conducted in the presenceof an aprotic solvent.
 28. The method of claim 27, wherein the aproticsolvent is selected from the group consisting of N,N-dimethylformamide,dimethyl sulfoxide, pyridine, and dioxane.
 29. The method of claim 26,wherein the condensing is conducted in the presence of a base.
 30. Themethod of claim 29, wherein the base is selected from the groupconsisting of 1,8-diazabicyclo[5.4.0]undec-7-ene, NaHCO₃, Na₂CO₃, andtriethylamine.
 31. The method of claim 26, wherein the condensing isconducted at a temperature of from about 25° C. to about 65° C.
 32. Themethod of claim 26, wherein R_(N) is an amino protecting group.
 33. Themethod of claim 32, wherein the amino protecting group forms a groupselected from the group consisting of an alkyl carbamate, allylcarbamate (Alloc), t-butyl carbamate (BOC), 9-fluorenylmethyl carbamate(FMOC), benzyl carbamate (Cbz), acetamide, chloroacetamide,trifluoroacetamide (TFA), phthalimide, benzylamine, triphenylmethylamine(tritylamine), benzylideneamine, p-toluenesulfonamide, and tosylamide.34. The method of claim 26, wherein W is oxygen (O).
 35. The method ofclaim 26, further comprising oxidizing an intermediate after condensing.36. The method of claim 35, wherein the oxidizing is conducted in thepresence of an oxidant selected from the group consisting of air,manganese dioxide, N-bromosuccinimide,1,8-diazabicyclo[5.4.0]undec-7-ene, N-bromosuccinimide together withbenzoyl peroxide, and N-bromosuccinimide together with1,8-diazabicyclo[5.4.0]undec-7-ene.
 37. The method of claim 35, whereinthe oxidizing is conducted in the presence of a solvent selected fromthe group consisting of dimethylformamide, tetrahydrofuran, dimethylsulfoxide, dichloromethane, and pyridine.
 38. The method of claim 35,wherein the oxidizing is conducted at a temperature of from about 30° C.to about 90° C.
 39. The method of claim 35, wherein the oxidizing isconducted without substantial isolation of the compound of Formula IVfrom a reaction mixture in which the compound of Formula IV wassynthesized.
 40. The method of claim 35, wherein the oxidizing comprisesadding an oxidant directly to the reaction mixture or a diluted reactionmixture comprising the reaction mixture diluted with solvent.
 41. Themethod of claim 35, further comprising, after the condensing and priorto the oxidizing, diluting the reaction mixture by an amount of at leastabout 2-fold.
 42. The method of claim 35, further comprising, after thecondensing and prior to the oxidizing, cooling the reaction mixture or adiluted reaction mixture comprising the reaction mixture diluted withsolvent from a condensation reaction temperature at which the condensingis conducted to a cooled temperature, wherein the cooled temperature isat least about 10° C. lower than the condensation reaction temperature.43. The method of claim 42, further comprising adding an oxidant to thereaction mixture or the diluted reaction mixture when the reactionmixture or the diluted reaction mixture is at the cooled temperature.44. The method of claim 43, further comprising, after the cooling,heating the reaction mixture or the diluted reaction mixture from thecooled temperature to a heated temperature and conducting the oxidizingat the heated temperature, wherein the heated temperature is at leastabout 10° C. higher than the cooled temperature.
 45. The method of claim42, wherein the oxidizing is conducted at the cooled temperature.
 46. Amethod of synthesizing a compound comprising condensing a compound ofFormula V to yield a framework containing an indole and thiazoline or aframework containing an indole and thiazole, wherein: Formula V is:

or a salt thereof; W and Y are each independently selected from thegroup consisting of oxygen (O) and sulfur (S); and R₁, R₂, R₃, R₄, R₅,and R_(N) are each independently selected from the group consisting ofhydrogen, deuterium, halo, amino, hydroxy, cyano, formyl, nitro, alkyl,haloalkyl, alkenyl, haloalkenyl, alkynyl, haloalkynyl, alkoxy,haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,carbonylthio, halocarbonylthio, thiocarbonyloxy, halothiocarbonyloxy,thiocarbonylthio, halothiocarbonylthio, and —S(O)_(n)R₈ (n=0 to 2, R₈ isdirectly connected to S), wherein R₈ is selected from the groupconsisting of hydrogen, deuterium, halo, amino, hydroxy, thiol, cyano,formyl, alkyl, haloalkyl, alkenyl, haloalkenyl, alkynyl, haloalkynyl,alkoxy, haloalkoxy, thioalkoxy, halothioalkoxy, alkanoyl, haloalkanoyl,thioalkanoyl, halothioalkanoyl, carbonyloxy, halocarbonyloxy,carbonylthio, halocarbonylthio, thiocarbonyloxy, halothiocarbonyloxy,thiocarbonylthio, and halothiocarbonylthio; and wherein the condensingis conducted in a protic solvent.
 47. The method of claim 46, whereinthe condensing is conducted in a solvent selected from the groupconsisting of an alcohol, an alcohol together with water, an alcoholtogether with an acid, an acid, an alcohol together with an aproticsolvent, or water together with an aprotic solvent.
 48. The method ofclaim 46, wherein the condensing is conducted at a temperature of about50° C. to about 70° C.
 49. The method of claim 46, wherein W and Y areeach oxygen (O).
 50. The method of claim 46, wherein W and Y are oxygen(O) and sulfur (S), respectively.
 51. The method of claim 46, whereinR_(N) is an amino protecting group.
 52. The method of claim 51, whereinthe amino protecting group forms a group selected from the groupconsisting of an alkyl carbamate, allyl carbamate (Alloc), t-butylcarbamate (BOC), 9-fluorenylmethyl carbamate (FMOC), benzyl carbamate(Cbz), acetamide, chloroacetamide, trifluoroacetamide (TFA),phthalimide, benzylamine, triphenylmethylamine (tritylamine),benzylideneamine, p-toluenesulfonamide, and tosylamide.